Focused on the issue that different blockchains are independent from and difficult to communicate with each other， a new type of permissioned public blockchain architecture of "main chain + sub chain" was proposed. Firstly， based on the existing algorithms such as Delegated Proof Of Stake （DPOS）， Verifiable Random Function （VRF） and Practical Byzantine Fault Tolerance （PBFT）， an innovative two-layer consensus algorithm was designed. And a trusted permission mechanism was added to make the blockchain have both permission and public characteristics. Secondly， the design process of the main and sub chains was described in detail. The management of the chain group and public services was provided by the main chain， while the sub chains were designed independently for different business scenarios， and cross-chain data communication was realized by connecting the main chain relay， thereby realizing the data secure isolation. Finally， an experimental environment was built for testing to verify the feasibility of the permissioned public blockchain design. Experimental results show that compared with some existing blockchains such as the Hyperledger Fabric， the proposed permissioned public blockchain has significant advantages， including a throughput of up to 25 000 times per second and an average delay time of about 8 s. It can be seen that this permissioned public blockchain provides technical support for further research on cross-chain data interconnection of different types of blockchains.

According to characters of marine ecology domain knowledge, a marine ecology knowledge organization model was proposed. Referring to engineering field literature and the device-function knowledge representation theory that the "function" concept was used to describe marine ecology functional process; a viewpoint of device-function was fixed, a domain upper ontology for marine ecosystem was presented, and then marine ecological conceptual model and marine ecology OWL ontology were constructed. By extending OWL-DL, OWL-Process model oriented function-process was proposed, and then marine ecology function-process ontology instance was constructed. Based on constructed marine ecology ontology repository, marine ecological knowledge management system was developed. The ontology application system provides marine ecology knowledge query and crisis early warning functions; and it also verifies the validity, rationality and feasibility of constructed marine ecology ontology.